Magic Stationary Phase

Magic stationary phase, is a spherical, completely porous silica gel. The manufacturing process permits no performance-reducing micropores, but yields constant specific surface area.  Magic packing materials have very high purity and are free from metallic contaminants that could hinder optimum peak shape.

Parameters of Magic packing material include silica gel composition, hydrophobic strength, steric selectivity, peak symmetry for basic analytes, silanol capacity and ion exchange sites. These parameters are determined by procedures such as NMR and ICP in addition to chromatographic techniques.  This multiplicity of characteristics yields extremely reproducible carrier materials providing a basis for reliable, constant, batch-independent, high-performance separation columns.

Ultra-Pure Silica Gel

The silica gel utilized in the manufacturing process of Magic packing material is 99.999% pure silica gel.  A particle size distribution analyzer is used to test each batch for constant particle size.  Surface area, pore size and pore volume are determined by a BET analyzer.  AAS and ICP are utilized to test for metallic impurities.  The extremely low level of metallic impurities guarantees that no sample will be adsorbed and no complexes will be formed during chromatographic analysis.

Bonding and End-Capping

Efficient bonding and the elimination of harmful silanol group residues is monitored by NMR.  The absence of silanol group residues is shown by the excellent chromatographic performance with respect to basic and acidic analytes.

PolySULFOETHYL Aspartamide

This strong cation-exchange (SCX-) material was developed by PolyLC, Inc. specifically for peptide applications.  At pH 2.0- 4.0, peptides lose their (-) charges, and have net positive charge.  They can be eluted from PolySULFOETHYL A columns with a salt gradient; elution is in order of absolute number of basic residues.  Thus, the selectivity complements that of reversed-phase chromatography (RPC).  The two modes, used in sequence, have completely resolved every crude mixture of peptides examined.  Thus, this combination is quite useful for peptide mapping or purification of peptides from crude tissue extracts.  It is also useful for purification of synthetic peptides.  It is convenient to use the SCX column first, since the capacity is several times (approx. >4 times) that of an equivalent RPC column; the collected fractions are then desalted during the subsequent RPC step.

Recovery of peptides from this column is high or quantitative.  This stands in contrast with the poor recovery often obtained with SCX columns in the past, which were based on sulfopropyl- (SP-) groups or aromatic sulfonates.  Hydrophobic binding is significant with such groups (unlike the sulfoethyl- group), and such binding is promoted by the salt gradients used for elution from ion-exchange columns, with attendant effects on recovery of hydrophobic polypeptides.

PLRP-S Columns

The PLRP-S packing material is a polymeric media consisting of poly(styrene/divinlybenzene).  PLRP-S packing material is manufactured by Polymer Laboratories.  It is specifically designed for Reversed-Phase separations of biomolecules.  The range of pore sizes enables high capacity/high resolution separations from the smallest peptide to the largest protein. In order to maximize selectivity and capacity, the high performance liquid chromatography (HPLC) media requires the maximum available surface area, yet with sufficiently large pores so that access is not restricted. 

The PLRP-S packing material is available in four different sizes.  This range includes Reversed Phase PLRP-S 5µ 100Å columns (small molecules, peptides) and PLRP-S 5µ 300Å columns (polypeptide/globular proteins), 8µ 1000Å columns (large fibrous proteins) and 8µ 4000Å columns (large biomolecules, large proteins).  We now offer 5µ particle size in the 1000Å and 4000Å pore size for protein analysis.

Outstanding Chemical Stability

PS/DVB macroporous packings, such as PLRP-S, are inherently and uniformly hydrophobic, and do not necessitate bonded ligands, unlike alkyl-functionalized silica reversed phase materials. Polymeric PLRP-S materials do not possess residual surface functionalities, and do not therefore suffer from the typical silica problems of acidic silanol groups or other ionic species, which can interfere with the separation performance of the matrix and are difficult to remove entirely and reproducibly by endcapping.

Unsurpassed pH Stability of Polymeric Media

PLRP-S columns are designed for easy use across the pH spectrum from 1 to 14, with none of the restrictions associated with silica packings. Polymeric packings offer the convenience of separations at the extremes of pH.

Higher Retentivity

PLRP-S media possesses a much greater surface area than alkyl-bonded silicas and therefore even polar molecules such as Parabens may be retained much longer, resulting in greater resolution. Parabens are esters of 4-hydroxybenzoic acid and are commonly used as preservatives in a variety of products, including cosmetics.

High Pressure / Flow Rate Stability

PLRP-S is produced by Polymer Laboratories using state-of-the-art manufacturing techniques. The high degree of crosslinking (divinylbenzene), together with optimized pore volume and structure, ensure that the spherical particles are more than rigid enough to withstand modern HPLC conditions. The formulation guarantees that pore size distribution falls within tightly controlled limits but still provides exceptionally high surface area for maximum resolution and binding capacity.

HALO

HALO C18 is a high-speed, high-performance liquid chromatography column based on a new Fused-Core^TM particle design.  The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core.  This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5-micron thick porous shell and the small overall particle size of 2.7-microns.  The densely bonded, extensively endcapped dimethyloctadecyl stationary phase of HALO C18 provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.  Halo material is manufactured by Advanced Materials Technology.

Magic Stationary Phase

Magic stationary phase, is a spherical, completely porous silica gel. The manufacturing process permits no performance-reducing micropores, but yields constant specific surface area.  Magic packing materials have very high purity and are free from metallic contaminants that could hinder optimum peak shape.

Parameters of Magic packing material include silica gel composition, hydrophobic strength, steric selectivity, peak symmetry for basic analytes, silanol capacity and ion exchange sites. These parameters are determined by procedures such as NMR and ICP in addition to chromatographic techniques.  This multiplicity of characteristics yields extremely reproducible carrier materials providing a basis for reliable, constant, batch-independent, high-performance separation columns.

Ultra-Pure Silica Gel

The silica gel utilized in the manufacturing process of Magic packing material is 99.999% pure silica gel.  A particle size distribution analyzer is used to test each batch for constant particle size.  Surface area, pore size and pore volume are determined by a BET analyzer.  AAS and ICP are utilized to test for metallic impurities.  The extremely low level of metallic impurities guarantees that no sample will be adsorbed and no complexes will be formed during chromatographic analysis.

Bonding and End-Capping

Efficient bonding and the elimination of harmful silanol group residues is monitored by NMR.  The absence of silanol group residues is shown by the excellent chromatographic performance with respect to basic and acidic analytes.

PolySULFOETHYL Aspartamide

This strong cation-exchange (SCX-) material was developed by PolyLC, Inc. specifically for peptide applications.  At pH 2.0- 4.0, peptides lose their (-) charges, and have net positive charge.  They can be eluted from PolySULFOETHYL A columns with a salt gradient; elution is in order of absolute number of basic residues.  Thus, the selectivity complements that of reversed-phase chromatography (RPC).  The two modes, used in sequence, have completely resolved every crude mixture of peptides examined.  Thus, this combination is quite useful for peptide mapping or purification of peptides from crude tissue extracts.  It is also useful for purification of synthetic peptides.  It is convenient to use the SCX column first, since the capacity is several times (approx. >4 times) that of an equivalent RPC column; the collected fractions are then desalted during the subsequent RPC step.

Recovery of peptides from this column is high or quantitative.  This stands in contrast with the poor recovery often obtained with SCX columns in the past, which were based on sulfopropyl- (SP-) groups or aromatic sulfonates.  Hydrophobic binding is significant with such groups (unlike the sulfoethyl- group), and such binding is promoted by the salt gradients used for elution from ion-exchange columns, with attendant effects on recovery of hydrophobic polypeptides.

PLRP-S Columns

The PLRP-S packing material is a polymeric media consisting of poly(styrene/divinlybenzene).  PLRP-S packing material is manufactured by Polymer Laboratories.  It is specifically designed for Reversed-Phase separations of biomolecules.  The range of pore sizes enables high capacity/high resolution separations from the smallest peptide to the largest protein. In order to maximize selectivity and capacity, the high performance liquid chromatography (HPLC) media requires the maximum available surface area, yet with sufficiently large pores so that access is not restricted. 

The PLRP-S packing material is available in four different sizes.  This range includes Reversed Phase PLRP-S 5µ 100Å columns (small molecules, peptides) and PLRP-S 5µ 300Å columns (polypeptide/globular proteins), 8µ 1000Å columns (large fibrous proteins) and 8µ 4000Å columns (large biomolecules, large proteins).  We now offer 5µ particle size in the 1000Å and 4000Å pore size for protein analysis.

Outstanding Chemical Stability

PS/DVB macroporous packings, such as PLRP-S, are inherently and uniformly hydrophobic, and do not necessitate bonded ligands, unlike alkyl-functionalized silica reversed phase materials. Polymeric PLRP-S materials do not possess residual surface functionalities, and do not therefore suffer from the typical silica problems of acidic silanol groups or other ionic species, which can interfere with the separation performance of the matrix and are difficult to remove entirely and reproducibly by endcapping.

Unsurpassed pH Stability of Polymeric Media

PLRP-S columns are designed for easy use across the pH spectrum from 1 to 14, with none of the restrictions associated with silica packings. Polymeric packings offer the convenience of separations at the extremes of pH.

Higher Retentivity

PLRP-S media possesses a much greater surface area than alkyl-bonded silicas and therefore even polar molecules such as Parabens may be retained much longer, resulting in greater resolution. Parabens are esters of 4-hydroxybenzoic acid and are commonly used as preservatives in a variety of products, including cosmetics.

High Pressure / Flow Rate Stability

PLRP-S is produced by Polymer Laboratories using state-of-the-art manufacturing techniques. The high degree of crosslinking (divinylbenzene), together with optimized pore volume and structure, ensure that the spherical particles are more than rigid enough to withstand modern HPLC conditions. The formulation guarantees that pore size distribution falls within tightly controlled limits but still provides exceptionally high surface area for maximum resolution and binding capacity.

HALO

HALO C18 is a high-speed, high-performance liquid chromatography column based on a new Fused-Core^TM particle design.  The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core.  This particle design exhibits very high column efficiency due to the shallow diffusion paths in the 0.5-micron thick porous shell and the small overall particle size of 2.7-microns.  The densely bonded, extensively endcapped dimethyloctadecyl stationary phase of HALO C18 provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.  Halo material is manufactured by Advanced Materials Technology.