Certified Protein Digest Standards
Certified protein tryptic digest standards are now available from eight species and one yeast protein mixture for use in HPLC, CE, LC/MS, CE/MS, ESI-MS and MALDI-MS instrument calibration. Pure protein standards are reduced with DTT, alkylated with IAA, and digested with trypsin. Digestions are monitored by HPLC to ensure complete digestion (>95%). The digests are then quantitated using HPLC/UV. 500 picomole aliquots of each digest are dried and stored at -20oC. Each protein digest batch is analyzed by LC-MS/MS, and resulting data searched to ensure a top match with good coverage. Each digest comes with a test certificate and instructions for digestion and storage.
Features
· Twelve protein tryptic digest test mixtures
· Batch test certificate
· Complete digestation (>95%)
· 6 Bovine Protein Digest Equal Molar Mix available
· 6 Bovine Protein Digest Exponential Mix available
Specifications
|
Species
|
Protein
|
Molecular Weight (kd)
|
PTM
|
|
Bovine
|
Beta Lactoglobulin
|
18.3
|
|
|
Bovine
|
Alpha Casein
|
23.6
|
(P)
|
|
Bovine
|
Carbonic Anhydrase
|
29.2
|
|
|
Bovine
|
Glutamate Dehydrogenase
|
55.5
|
|
|
Bovine
|
Serum Albumin
|
69.3
|
|
|
Bovine
|
Lactoperoxidase
|
79.8
|
(G)
|
|
Equine
|
Cytochrome C
|
11.8
|
|
|
Equine
|
Apo-Myoglobin
|
16.9
|
|
|
Human
|
Alpha Lactalbumin
|
16.2
|
(G)
|
|
Human
|
Acid Glycoprotein
|
23.5
|
(G)
|
|
Yeast
|
Alcohol Dehydrogenase
|
36.8
|
(P)
|
|
Yeast
|
Enolase
|
46.8
|
(P)
|
Certified Protein Digest Standards
Certified protein tryptic digest standards are now available from eight species and one yeast protein mixture for use in HPLC, CE, LC/MS, CE/MS, ESI-MS and MALDI-MS instrument calibration. Pure protein standards are reduced with DTT, alkylated with IAA, and digested with trypsin. Digestions are monitored by HPLC to ensure complete digestion (>95%). The digests are then quantitated using HPLC/UV. 500 picomole aliquots of each digest are dried and stored at -20oC. Each protein digest batch is analyzed by LC-MS/MS, and resulting data searched to ensure a top match with good coverage. Each digest comes with a test certificate and instructions for digestion and storage.
Features
· Twelve protein tryptic digest test mixtures
· Batch test certificate
· Complete digestation (>95%)
· 6 Bovine Protein Digest Equal Molar Mix available
· 6 Bovine Protein Digest Exponential Mix available
Specifications
|
Species
|
Protein
|
Molecular Weight (kd)
|
PTM
|
|
Bovine
|
Beta Lactoglobulin
|
18.3
|
|
|
Bovine
|
Alpha Casein
|
23.6
|
(P)
|
|
Bovine
|
Carbonic Anhydrase
|
29.2
|
|
|
Bovine
|
Glutamate Dehydrogenase
|
55.5
|
|
|
Bovine
|
Serum Albumin
|
69.3
|
|
|
Bovine
|
Lactoperoxidase
|
79.8
|
(G)
|
|
Equine
|
Cytochrome C
|
11.8
|
|
|
Equine
|
Apo-Myoglobin
|
16.9
|
|
|
Human
|
Alpha Lactalbumin
|
16.2
|
(G)
|
|
Human
|
Acid Glycoprotein
|
23.5
|
(G)
|
|
Yeast
|
Alcohol Dehydrogenase
|
36.8
|
(P)
|
|
Yeast
|
Enolase
|
46.8
|
(P)
|