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The Paradigm Nano-Capillary MDLC is a fully configurable high-performance instrument that is designed to provide precise, reproducible separations for proteomics LC/MS. Available in bio-inert titanium, each MDLC in the Paradigm NC family features integrated piston seal wash, 10-port valve, automatic dynamic-adjusting active splitter, and low-volume mixing for rapid delivery of the gradient from the pump to the columns. Paradigm MDLC provide unsurpassed reproducible separations from 50 nL/min to 5 mL/min.
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Features:
• 2 to 4 Independent pumps
• 1 to 2 Independent mixers
• 2 to 4 Independent pressure
transducers
• 1 to 2 Independent 10-port valves
• Full software control
• Michrom Paradigm
• Thermo Xcalibur
• ABI/Sciex Analyst
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•Automated PistonWash
• 50 nL/min – 5 mL/min flow
• Completely bio-inert fluidics
• Automated purging
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Paradigm MS2-NC
The Paradigm MS2-NC is a binary high-pressure mixing MDLC commonly used in proteomics as a premier front-end to the majority of commercially available mass spectrometers. The high precision gradient formation provides highly reproducible separations with even the most aggressive buffers or mobile phase composition. The MS2 is ideal for high-throughput ballistic gradient quantitative analysis by LC MRM-MS/MS. Learn more...
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24 hour reproducibility of 1 fmol BSA tryptic digest at 500 nL/min
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Paradigm MS3-NC
The Paradigm MS3-NC high-pressure mixing MDLC comprises three integrated high-pressure pumps. High speed sample loading is achieved with one pump while the other two complete binary gradient runs with the integrated 10-port valves. Paradigm MS3-NC systems are typically coupled with the Paradigm HTC PAL for automated sample fractionation, digestion, pretreatment and including enrichment, desalting, or detergent removal prior to injection. Learn more...
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96 spots from a prostate cancer cell lysate 2D gel cut, auto-digested with trypsin, and analyzed using a MS3-NC; resulting in 96 MS/MS data files identifying new protein biomarkers.
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Paradigm MS4-NC
The Paradigm MS4 featuring four pumps, two independent mixers, and two 10-port valves is the flagship of the Paradigm line. The MS4 is ideal for automated 1D – 4D chromatography with fractionation of complex proteome samples (proteins or peptides) prior to LCMS analysis. The MS4-NC is often used for automated online or offline Mudpit. Coupling the MS4-NC with the Michrom ADVANCE CaptiveSpray source provides higher speed, sensitivity, and robust operation for unattended overnight and weekend runs without the risk of source plugging or fouling. Learn more...
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96 replicate injections of protein biomarkers in complex proteome samples
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